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Inflammatory Bowel Disease (IBD) Models

Inflammatory Bowel Diseases are characterized by long term inflammation of the gut and includes two types of states: ulcerative colitis (UC) and Crohn’s disease (CD). Symptoms of these disease are stomach pain, bloody diarrhea, weight loss, etc.

The causes of IBD are still unclear but gut microbiota, genetic disorders, defects in immune system, and/or environmental factors have been shown to play a role.

Using chemical agents, TransCure bioServices has developed two IBD mouse models.

Chemically induced DSS mouse models:

This mouse model mimics ulcerative colitis. The Dextran Sodium Sulfate (DSS) destroys the mucus layer and disrupts the epithelial barrier, inducing activation of propria immune cells with the influx of luminal bacteria. This model induces the secretion of pro-inflammatory cytokines such as IL-6 or TNF-alpha.

DSS acute: experimental design

DSS Mouse Model

Readouts

  • Daily monitoring (clinical score, diarrhea, rectal bleeding, BW loss)
  • Endoscopy (max 2x/week)
  • Colon length and weight
  • Histology (inflammation, mucosal damage, cell infiltration)
  • Survival

Chemically induced TNBS mouse models:

This mouse model mimics Crohn’s disease. Intra-rectal instillation of TNBS in ethanol disrupts the mucosal barrier, allowing TNBS to bind to colon proteins. Haptenized proteins are processed by antigen-presenting cells in the lamina propria, inducing excessive secretion of IL-12 and pro-inflammatory cytokines.

TNBS acute: experimental design

TNBS Mouse Model

Readouts

  • Daily monitoring (clinical score, diarrhea, rectal bleeding, BW loss)
  • Endoscopy (max 2x/week)
  • Colon length and weight
  • Histology (inflammation, mucosal damage, cell infiltration)
  • Survival

Interested to know more? Do not hesitate to contact us!

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