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CD34+ Humanized Mouse

Advanced Human Immune System Mouse Models™

Humane Immune System Humanized MouseThe immune system plays a central role in many pathologies such as cancer or inflammatory and autoimmune diseases. Many drug candidates, targeting the immune system, have therefore been developed, revealing the need for predictive pre-clinical models in the field.

TransCure bioServices has developed a highly predictive mouse model with a human immune system. Highly deficient mice are transplanted with CD34+ hematopoietic stem cells from umbilical cord blood. Complete hematopoiesis is fully recapitulated, and 14 weeks after CD34+ engraftment, a fully functional human immune system is established.

Mouse Humanization Process

Mouse Humanization Process

The main characteristics of this humanized mouse model are:

  • Development of a complete and functional human immune system. By comparison to human PBMC engrafted mice, CD34+ humanized mice support the development not only of T cells but also B cells, NK cells, dendritic cells, monocytes and macrophages, allowing testing of many different drug mecanisms of action such as T-cell engagers, ADCC, macrophage polarization…
  • All the mouse tissues are infiltrated (peripheral blood, spleen, bone marrow…)
  • Poor GvHD onset thanks to high purity of CD34+ hematopoietic cells
  • Lifelong stability (>6 months) allowing long term studies
  • Highly standardized process: we guarantee a humanization rate above 25% (in average 40% of humanization)
  • CD34+ Humanized mice are always in stock. There are no wait times to start your study. We produce over 700 humanized mice every month
  • Due to the lack of human cytokines in the system, CD34+ humanized mice are characterized by lower myeloid lineage development and NK cells activity. To overcome this limitation, we have developed the human cytokines boost. Via hydrodynamic gene delivery, we enhance the proliferation and maturation of specific human immune subsets. A cocktail of GM-CSF, IL-3, IL-4 and FLT3L increase the number of monocytes and dendrictic cells. A IL-15 boost increase the number of NK cells and their activity

Myeloid lineage, DC and NK cells Boosting

Myeloid lineage, DC and NK cells Boosting